Review on Microbial Sources, Production, Purification and Potential Industrial Applications of Laccases

Authors

  • Amit Rastogi Research Scholar, Shri Venkateshwara University, Gajraula, U.P., INDIA
  • Rajesh Singh Director Research and Planning, Shri Venkateshwara University, Gajraula, U.P., INDIA
  • Ahmed Barhoum Associate Professor, Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt and School of Chemical Sciences, Dublin City University, Dublin, IRELAND

Keywords:

Microbial Sources, Purification, Potential Industrial Applications, Laccases, biosensor development, agriculture

Abstract

Laccase (EC 1.10.3.2) is a multicopper blue oxidase which are involved in the oxidation of a broad range of organic substrates, including phenols, polyphenols, anilines, and even certain inorganic compounds by a one-electron transfer mechanism. Laccases are widely distributed in bacteria, fungai, insects and higher plants. There are mainly two production techniques for cultivation of laccase such as submersed fermentation and solid- state fermentation. This paper briefly discuss the effect of carbon source, effect of nitrogen source, effect of inducers, effects of surfactants, effect of agitator, influence of metal ions and  use of agro-industrial waste in production medium. The paper also discussed the purification techniques such as ammonium sulphate precipitation for extraction purpose followed by dialysis and ion-exchange chromatography as well characterization techniques. Laccases are known to show application ranging from pharmaceutical industries to textile sector as well as in biosensor development.

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DOI: 10.47856/ijaast.2021.v08i12.004

Author Biographies

Amit Rastogi, Research Scholar, Shri Venkateshwara University, Gajraula, U.P., INDIA

Research Scholar, Shri Venkateshwara University, Gajraula, U.P., INDIA

Rajesh Singh, Director Research and Planning, Shri Venkateshwara University, Gajraula, U.P., INDIA

Director Research and Planning, Shri Venkateshwara University, Gajraula, U.P., INDIA

Ahmed Barhoum, Associate Professor, Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt and School of Chemical Sciences, Dublin City University, Dublin, IRELAND

Associate Professor, Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt and School of Chemical Sciences, Dublin City University, Dublin, IRELAND

Published

2021-12-27